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KMID : 0043319800030010017
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Archives of Pharmacal Research
1980 Volume.3 No. 1 p.17 ~ p.21
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Spectrophotofluorometric Analysis of 2-[[3-(Trifluoromethyl) phenyl]amino]-3-pyridine Carboxylic Acid in Urine
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Lee KC
Min SH/Rhee SH/Lee YJ
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Abstract
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A sensitive spectrophotofluorometric method was developed for the analysis of 2-[[3-(trifluoromethyl) phenyl]amino]-3-pyridine carboxylic acid (I) in urine. The method is based on the fluoroscence behavior of the I-aluminum complex in absolute ethanol. This fluorophore has activation and emission wavelengths of 355 and 450nm, respectively. Optimum conditions for the reaction were investigated. The fluorescence was linear in the range of 0.25 3.0mcg of I/ml. Replicate studies of spiked urine samples, each containing 2.5mcg of I/ml showed good precision with a relative standard deviation of 0.019. Overall recovery percent from five spiked urine samples was 99.4 +/- 1.32%.
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